Sterilized water in glass jar

Description

Introducing Our 300ml Sterilized water in glass jar with butyl rubber injection port. 

Our 300ml Injection Jars come pre-filled with distilled and sterilized water, making them perfect for creating your own spore solution. Simply scrape your spore print into the jar and securely screw the lid back on. With the injection port, you can easily draw out the spore solution using a 21g needle. Test your solution on agar to ensure cleanliness, and once confirmed, you’ll have a spore solution ready to use for your mushroom cultivation needs.

Key Features:

• Sterile and Ready: Pre-filled with distilled water for easy spore suspension.
• Injection Port: Allows easy withdrawal with a 21g needle.
• Reusable: Re-sterilize the jar after each use for future projects.

How to Use:

1. Scrape your spore print into the jar under sterile conditions.
2. Shake the jar to create a spore solution.
3. Test the solution on an agar plate to ensure it’s free of contaminants.
4. Store the jar in a fridge for future use, securing with micro pore tape for extra protection.

This sterilized water in glass jar is ideal for cultivators looking for a reliable and reusable method to prepare, store, and test their spore solutions.

Experiment: Making a Spore Solution Jar and Spore Syringe at Home

Materials Needed:

• spore print
• 300ml sterilized water in a glass jar with injection port
• Sterile gloves
• 21g sterile syringe and needle
• Alcohol swabs
• Flame or alcohol lamp
• Agar plate (for testing spore solution)

Procedure:

1. Prepare the Environment: Sterilize your workspace by wiping down all surfaces with alcohol and work in a still air box if available. Wear sterile gloves to avoid contamination.
2. Open the Jar: Carefully unscrew the lid of the sterilized jar under sterile conditions. Ensure that the jar remains clean and free of any contaminants.
3. Add Spore Print: Using a sterile inoculation loop or a sterilized blade, scrape a small portion of the spore print into the jar. Avoid touching the inner rim of the jar to keep it sterile.
4. Seal the Jar: Immediately screw the lid back on the jar to prevent contamination. Gently shake the jar to evenly disperse the spores in the water, creating a spore solution.
5. Test Spore Solution: To ensure the solution is clean, remove a small amount using a 21g syringe. Wipe the injection port with an alcohol swab, then inject the spore solution onto an agar plate. Incubate the agar plate at 25-26°C to check for mycelium growth or contamination.
6. Create Spore Syringe: After confirming that the spore solution is clean, sterilize a 21g syringe. Wipe the jar’s injection port with alcohol and use the syringe to draw out the desired amount of spore solution.
7. Store the Solution: After use, reseal the jar and cover the injection port with micro-pore tape to ensure it stays clean. Store the jar in a fridge (2-8°C) for future use.

Results: If the spore solution tests clean on agar, you now have a homemade spore syringe ready for inoculation in your mushroom-growing process! You can use this spore syringe to inoculate sterilized substrate jars or bags for mycelium cultivation.